At Technical University of Denmark, the tuition fee is 25,000 USD per year.
This course provides a comprehensive introduction to the essential analytical techniques used to identify and quantify small organic molecules and proteins. Participants will learn how these biomolecules are extracted, prepared, and analyzed using advanced chromatographic and mass spectrometric methods. With a strong emphasis on data interpretation and compound identification—both known and unknown—the course is designed for anyone working in biotechnology, health sciences, or analytical chemistry who wants to build or strengthen their expertise in modern bioanalytical workflows. Whether you’re a PhD student or an industry professional, you’ll gain practical skills that are directly applicable to research, product development, and quality control. Content The course program: Day 1: Course introduction Project intro Intro to Mass Spectrometry (MS) Data processing (Split) Intro to Metabolomics (SJ) Targeted metabolomics: Data analysis (workshop) (SJ) Best practices in metabolomics: QA and QC Day 2: Intro to metabolomics and proteomics Instruments and LC/GC Advanced metabolomics: Molecular networking and in silico Identification Spatial metabolomics Data interpretation and wrap-up Day 3: Untargeted metabolomics: Data Preprocessing with MZmine (Workshop) (SJ) Detectors Data types and format PTMs: phospho (CF) Project work Day 4: Intro and sample prep proteomics (ES) Data acquisition Proteins vs metabolites Metabolite annotation and data repositories, FAIR principles (SJ) Visualization, interpretation, and downstream analysis (Split) (AS) Project work Day 5: PTMs continued: Glyco and proteolysis Targeted proteomics Activity-based profiling Interactomics, spatial proteomics Meta & Paleo proteomics Quantification SILAQ TMT/iCAT/ITRAQ/LFQ Single cell proteomics De novo peptide sequencing, Immunopeptidomics AI in proteomics and protein science Proteomics data acquisition and processing Data processing, statistics, and data analysis Proteomics and metabolomics core infrastructures and capabilities Student presentations Learning outcomes After the course you will be able to: Describe the general principles of sample preparation of small organic molecules and proteins, e.g. using solid phase extraction (SPE). Explain the general principle of chromatography (LC, GC) including the use of different relevant types of columns. Explain the general principles of diode array detection (DAD) and mass spectrometry (e.g. QTOF) coupled to liquid chromatography (LC-DAD-MS). Demonstrate the principles of dereplication and accurate mass spectrometry. Perform data analysis using standard instrumental software and databases for LC-DAD-MS analysis. Compare analytical results to information in commercial and open source databases. Explain the basic principles behind quantitative analysis of small organic molecules. Explain the principles behind mass spectrometry based (e.g. Orbitrap and MALDI TOF) analysis of proteins. Describe common methodology in proteomics workflows. Understand the various ways of conducting quantitative proteomics, e.g label-free vs. labeled. Gain insight into MS-based proteomics data processing. Who should attend? PhD Students and Researchers working in work involves mass spectrometry, metabolite or protein analysis, or developing analytical methods for biological or chemical research Research scientists and lab technicians Analytical chemists and bioanalytical method developers Process development engineers QC/QA specialists working with complex biomolecular products Scientific staff transitioning from traditional wet-lab work to omics-based technologies Important information Here you will find important information for short course participants regarding cancellation policies, location and waitlist policies. The lectures, laboratory exercises and group work will all be conducted physically at DTU Lyngby Campus. Admission requirements Participants do not need to have specific knowledge in the field of biotechnology other than a basic high-school level understanding of natural sciences. Fee, registration and location NB! DTU PhD students must register for this course through the Study Planner PhDs from other universities must register as a guest student through: https://www.dtu.dk/english/education/phd/intro/guest-phd/guest_courses/registration_form The application deadline is two weeks prior to course start. DTU campus Lyngby Discounts Employees in start-up companies, educational institutions and the public sector a 50% course fee reduction. Course coordinator Thomas Ostenfeld Larsen Senior Researcher Email: tol@bio.dtu.dk Phone number: +45 45252632 Read more Erwin Schoof Associate Professor Email: erws@dtu.dk Phone number: (+45) 4525 2059 Read more Konstantinos Kalogeropoulos Assistant Professor Email: konka@dtu.dk Read more Scott Alexander Jarmusch Assistant Professor Email: salja@dtu.dk Phone number: (+45) 45252570 Read more
